2FIW
Crystal Structure of the GCN5-Related N-acetyltransferase: Aminotransferase, Class-II from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 78.293, 78.293, 93.242 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.630 - 2.350 |
| R-factor | 0.22335 |
| Rwork | 0.219 |
| R-free | 0.26271 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.075 | 0.670 |
| Number of reflections | 13907 | |
| <I/σ(I)> | 11.3 | 5.4 |
| Completeness [%] | 97.8 | 98.5 |
| Redundancy | 16 | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2M Ammonium Sulphate, 2% PEG400, 0.1 M Hepes Na pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
