2FIU
Crystal Structure of the Conserved Protein of Unknown Function ATU0297 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.835, 41.835, 197.502 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.000 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.26440 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.922 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.116 | 0.473 |
| Number of reflections | 14348 | |
| <I/σ(I)> | 6.5 | 1.81 |
| Completeness [%] | 88.9 | 46.4 |
| Redundancy | 21.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 0.1 M Tris pH 6.6, 0.2 M Magnessium Nitrate, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
