2FI0
The crystal structure of the conserved domain protein from Streptococcus pneumoniae TIGR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | H 3 |
Unit cell lengths | 92.420, 92.420, 41.343 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.230 - 2.100 |
R-factor | 0.20438 |
Rwork | 0.203 |
R-free | 0.23627 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.277 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.155 |
Number of reflections | 6747 | |
<I/σ(I)> | 13.77 | 3.2 |
Completeness [%] | 91.6 | 43.5 |
Redundancy | 3 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 2.5 M NaCl, 0.1M Tris-HCl, 0.2M MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |