2FHZ
Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2003-03-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 61.790, 73.640, 110.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.150 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.20000 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.296 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.200 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.034 | 0.175 |
Number of reflections | 82619 | |
<I/σ(I)> | 20.1 | 9.1 |
Completeness [%] | 92.7 | 82.8 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 50 % saturated ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 50 % saturated ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |