2FHX
Pseudomonas aeruginosa SPM-1 metallo-beta-lactamase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-26 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97948 |
Spacegroup name | H 3 |
Unit cell lengths | 126.936, 126.936, 90.461 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
Rwork | 0.194 |
R-free | 0.24700 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.232 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.05) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.063 | 0.027 | 0.599 |
Number of reflections | 42802 | ||
Completeness [%] | 99.9 | 98.8 | 100 |
Redundancy | 5.6 | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris.Cl, 2.0M ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris.Cl, 2.0M ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris.Cl, 2.0M ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |