2FHT

Crystal Structure of Viral Macrophage Inflammatory Protein-II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-13
Detector	CUSTOM-MADE
Wavelength(s)	0.9559
Spacegroup name	P 41 2 2
Unit cell lengths	45.750, 45.750, 66.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.242
Rwork	0.239
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cm9
RMSD bond length	0.010
RMSD bond angle	1.589
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.058	0.688
Number of reflections	5987
Completeness [%]	98.6	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.1 M sodium citrate, 11% (w/v) PEG 4000, 11%(w/v) 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.1 M sodium citrate, 11% (w/v) PEG 4000, 11%(w/v) 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K