2FHQ
Crystal Structure of General Stress Protein from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.223, 66.280, 50.690 |
| Unit cell angles | 90.00, 114.77, 90.00 |
Refinement procedure
| Resolution | 26.890 - 1.870 |
| R-factor | 0.1991 |
| Rwork | 0.193 |
| R-free | 0.25347 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.087 | 0.352 |
| Number of reflections | 19822 | |
| <I/σ(I)> | 10.7 | 2.34 |
| Completeness [%] | 90.5 | 53.4 |
| Redundancy | 7.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.20M magnesium chloride, 0.1M bis-tris pH 5.5, 25%w/v polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
