2FHL
avidin related protein (AVR4)-BNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-06-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.039, 78.039, 110.028 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.050 |
| R-factor | 0.16711 |
| Rwork | 0.167 |
| R-free | 0.17708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y52 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 157254 | |
| <I/σ(I)> | 34.7 | |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 3.0M format, 0.1 M acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
