2FHD
Crystal structure of Crb2 tandem tudor domains
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.924, 116.924, 87.015 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.000 - 2.400 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.190 | 0.082 |
| Number of reflections | 25798 | |
| <I/σ(I)> | 35.4 | 3.11 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.3 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES-Na, 0.8M NaH2PO4, 0.8M KH2PO4, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
