2FH9
Structure and dimerization of the kinase domain from yeast Snf1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Wavelength(s) | 0.9686, 0.9796, 0.9794 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 108.677, 108.677, 61.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.336 |
Rwork | 0.215 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.383 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.02) |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 8763 |
Completeness [%] | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |