2FGZ
Crystal Structure Analysis of apo pullulanase from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.71 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.984, 60.214, 132.791 |
Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
Resolution | 14.940 - 1.750 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.21400 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.384 |
Number of reflections | 108442 | |
<I/σ(I)> | 10.4 | 6.8 |
Completeness [%] | 98.6 | 99.7 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15-25% polyethyleneglycol 6000 in 0.2M magnesium acetate, 0.2M sodium acetate buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |