2FGP
Crystal structure of a minimal, all RNA hairpin ribozyme with modifications (g8dap, u39c) at ph 8.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 83 |
Detector technology | CCD |
Collection date | 2005-05-05 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.770, 93.770, 127.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.370 - 2.400 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.25900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ZFR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | CRYSTAL (CLEAR) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS (1.1) |
Refinement software | CNS (V1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.010 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.034 | 0.458 |
Number of reflections | 12161 | |
<I/σ(I)> | 34.8 | 5.1 |
Completeness [%] | 89.8 | 92.8 |
Redundancy | 10.56 | 9.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | PEG2000 MME, LITHIUM SULFATE, SPERMIDINE, COBALT HEXAMMINE, pH 8.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG2000 MME | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | LITHIUM SULFATE | ||
3 | 1 | 1 | SPERMIDINE | ||
4 | 1 | 1 | COBALT | ||
5 | 1 | 1 | HEXAMMINE | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG2000 MME | ||
8 | 1 | 2 | LITHIUM SULFATE | ||
9 | 1 | 2 | COBALT |