2FGO

Structure of the 2[4FE-4S] ferredoxin from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-07-23
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	39.087, 45.129, 51.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1blu
RMSD bond length	0.009
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.350
High resolution limit [Å]	1.320	1.320
Rmerge	0.064	0.373
Number of reflections	20405
<I/σ(I)>	8.9	2
Completeness [%]	91.8	100
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4	277	3.2M (NH4)2HPO4, 0.230M NaCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.40