2FGN
Structural Studies Examining the Substrate Specificity Profiles of PC-PLCBc Protein Variants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2003-12-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.522, 90.522, 70.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.040 |
R-factor | 0.22 |
Rwork | 0.179 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.054 | 0.038 | 0.110 |
Number of reflections | 19621 | ||
Completeness [%] | 96.5 | 96 | 95.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 45% saturated ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |