2FGC
Crystal structure of Acetolactate synthase- small subunit from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.782, 104.782, 78.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.260 - 2.300 |
| R-factor | 0.17663 |
| Rwork | 0.168 |
| R-free | 0.23643 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.847 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.260 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.458 |
| Number of reflections | 9510 | |
| <I/σ(I)> | 35.89 | 4.6 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% V/V GLYCEROL, 0.4% W/V NDSB201, 0.15M MG FORMATE, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
