2FGB

Crystal structure of human 17bet a-hydroxysteroid dehydrogenase type 5 in complexes with PEG and NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	55.519, 61.653, 95.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.570 - 1.350
Rwork	0.172
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.255
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.570
High resolution limit [Å]	1.350
Number of reflections	68644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	30% PEG 4000, 0.1 M sodium citrate, 0.24 M ammonia sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K