2FFM
X-Ray Crystal Structure of Protein SAV1430 from Staphylococcus aureus. Northeast Structural Genomics Consortium Target ZR18.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 40.235, 40.235, 122.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.710 - 2.510 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.710 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | 0.147 |
| Number of reflections | 5872 | |
| <I/σ(I)> | 37.6 | 13.47 |
| Completeness [%] | 89.6 | 99.8 |
| Redundancy | 8.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 100 mM tri-sodium citrate dihydrate, 1.0 M mono-Ammonium dihydrogen Phosphate, and 5 mM DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
