2FFF
Open Form of a Class A Transpeptidase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.661, 78.043, 60.315 |
| Unit cell angles | 90.00, 113.02, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.230 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bg1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.326 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.350 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 27505 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Tris, 20% PEG MME 2000, 10 mM NiCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
