2FF7
The ABC-ATPase of the ABC-transporter HlyB in the ADP bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.370, 34.837, 37.820 |
Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.1944 |
Rwork | 0.192 |
R-free | 0.23107 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mt0 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.434 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 26698 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 90.5 | 69.5 |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM Tris, 10% PEG 6000, 5% MPD, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |