2FF4
Mycobacterium tuberculosis EmbR in complex with low affinity phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.390, 82.040, 81.080 |
| Unit cell angles | 90.00, 115.37, 90.00 |
Refinement procedure
| Resolution | 46.000 - 1.900 |
| R-factor | 0.21644 |
| Rwork | 0.215 |
| R-free | 0.23941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.294 |
| Number of reflections | 75792 | |
| <I/σ(I)> | 15 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris-HCl, 2.5% PEG 8000, 3% ethylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
