2FEX
The Crystal Structure of DJ-1 Superfamily Protein Atu0886 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.411, 89.411, 163.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.330 - 1.700 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.330 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.093 | 0.573 |
| Number of reflections | 73282 | |
| <I/σ(I)> | 51.571 | 3.463 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 19 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris 8.5, 1.5M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
