2FEF
The Crystal Structure of Protein PA2201 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 118.588, 118.588, 144.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.900 |
| R-factor | 0.18743 |
| Rwork | 0.186 |
| R-free | 0.22201 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.431 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.239 |
| Number of reflections | 76453 | |
| <I/σ(I)> | 33.945 | 3.98 |
| Completeness [%] | 98.8 | 99.14 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M K Formate, 22% PEG 3350, 4% Dioxan, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
