2FEE
Structure of the Cl-/H+ exchanger CLC-ec1 from E.Coli in NaBr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9198 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.822, 97.684, 170.202 |
| Unit cell angles | 90.00, 131.11, 90.00 |
Refinement procedure
| Resolution | 128.040 - 3.200 |
| R-factor | 0.25357 |
| Rwork | 0.251 |
| R-free | 0.29257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.466 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.040 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.071 | 0.523 |
| Number of reflections | 43955 | |
| <I/σ(I)> | 19.1 | 2.3 |
| Completeness [%] | 99.0 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM NaBr, PEG 400 37% , 0.05 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
