2FEC
Structure of the E203Q mutant of the Cl-/H+ exchanger CLC-ec1 from E.Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.9198 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.311, 98.496, 170.415 |
| Unit cell angles | 90.00, 131.51, 90.00 |
Refinement procedure
| Resolution | 128.040 - 3.967 |
| R-factor | 0.27184 |
| Rwork | 0.269 |
| R-free | 0.32555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 128.040 |
| High resolution limit [Å] | 3.967 |
| Rmerge | 0.085 |
| Number of reflections | 23320 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 250 mM NaBr, PEG 400 37%, 0.05 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
