2FE8
SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.872, 103.065, 91.728 |
| Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22900 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.11) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.083 | 0.044 | 0.536 |
| Number of reflections | 103572 | ||
| <I/σ(I)> | 30.5 | 2.2 | |
| Completeness [%] | 97.6 | 100 | 81.2 |
| Redundancy | 7 | 7.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 295 | 1.3 M ammonium sulfate, 100 mM sodium citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.20 |
