2FE7
The crystal structure of a probable N-acetyltransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.375, 52.036, 58.970 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 70.530 - 2.000 |
| R-factor | 0.18924 |
| Rwork | 0.188 |
| R-free | 0.22124 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.172 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.530 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.357 |
| Number of reflections | 27694 | |
| <I/σ(I)> | 17.1 | 2.32 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.1M Tris, 0.02M CaCl2, 20% PEG3350, 3% 1,6 Hexandiol, 3.3% sucrose, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
