2FE6
P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-07-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.390, 64.390, 252.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.500 |
| R-factor | 0.236 |
| Rwork | 0.227 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1yrd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.340 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 84598 | 18162 |
| <I/σ(I)> | 7.01 | 2.38 |
| Completeness [%] | 96.6 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 7.5 MG/ML P450, 50 MM TRIS, 125 MM KCL, 13.5-15% PEG8000, 50 MM DTE, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
