2FE5
The Crystal Structure of the Second PDZ Domain of Human DLG3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.487, 40.242, 33.340 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 26.450 - 1.100 |
R-factor | 0.11512 |
Rwork | 0.113 |
R-free | 0.15038 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2BYG.pdb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.439 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.450 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 27971 | |
Completeness [%] | 98.4 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Ammonium sulphate, Glycerol, Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |