2FE3
The crystal structure of bacillus subtilis PerR-Zn reveals a novel Zn(Cys)4 Structural redox switch
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976302, 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.290, 42.160, 53.490 |
| Unit cell angles | 82.18, 80.11, 60.96 |
Refinement procedure
| Resolution | 36.790 - 1.750 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.22900 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.229 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.087 | 0.417 |
| Number of reflections | 29824 | |
| <I/σ(I)> | 11.31 | 3.5 |
| Completeness [%] | 89.3 | 83.7 |
| Redundancy | 2.4 | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 25% PEG 6000, 1.5 M LiCl, 0.1 M Tris-HCl, pH 8.0, temperature 293K |
