2FDN
2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDI-URICI
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-01 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 33.950, 33.950, 74.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.940 |
R-factor | 0.1003 |
Structure solution method | TAKEN FROM PDB ENTRY 1FDN |
RMSD bond length | 0.027 |
RMSD bond angle | 0.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.950 |
High resolution limit [Å] | 0.940 | 0.940 |
Rmerge | 0.073 | 0.483 |
Total number of observations | 47452 * | |
Number of reflections | 28084 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 98.7 | 93.7 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 281 * | 55-60% AMMONIUM SULFATE, 100 MM TRISMALEATE PH 6.6-7.0 MIXED 1:1 WITH 10 MG/ML PROTEIN SOLUTION. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 55-60 (%sat) | |
3 | 1 | drop | TrisMaleate | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 55-60 (%sat) | |
5 | 1 | reservoir | TrisMaleate | 100 (mM) |