2FD5
The crystal structure of a transcriptional regulator from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.411, 71.411, 147.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.900 - 1.700 |
| R-factor | 0.21265 |
| Rwork | 0.211 |
| R-free | 0.24166 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.171 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.900 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.096 | 0.556 |
| Number of reflections | 23774 | |
| <I/σ(I)> | 27.1 | 1.58 |
| Completeness [%] | 98.8 | 82.4 |
| Redundancy | 12.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | PEG2KMME 30%, NaCitr. 0.1M, NaAcetate 0.2M, Tacsimate 1%, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
