2FD2

CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	55.200, 55.200, 95.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.990 *
Rwork	0.232
RMSD bond length	0.017
RMSD bond angle	3.650
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.990 *
Rmerge	0.121 *
Total number of observations	108979 *
Number of reflections	9084 *
Completeness [%]	86.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1 (mM)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop		4.8 (M)
4	1	drop		1 (mM)	for crystals mounted anaerobically