2FCZ

HIV-1 DIS kissing-loop in complex with ribostamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-01
Detector	MARRESEARCH
Wavelength(s)	0.91946
Spacegroup name	C 1 2 1
Unit cell lengths	112.125, 27.191, 106.909
Unit cell angles	90.00, 116.72, 90.00

Refinement procedure

Resolution	19.170 - 2.010
R-factor	0.238
Rwork	0.238
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fcx
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.010	2.010
Number of reflections	18764
<I/σ(I)>	18.9	5.7
Completeness [%]	95.4	99.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	310	MPD, KCl, MgCl2, Na Cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K