2FCY

HIV-1 DIS kissing-loop in complex with Neomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-01
Detector	MARRESEARCH
Wavelength(s)	0.91946
Spacegroup name	C 2 2 21
Unit cell lengths	27.105, 113.702, 96.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.220 - 2.200
R-factor	0.235
Rwork	0.235
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fcx
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.200	2.200
Number of reflections	7689
<I/σ(I)>	20.7	6.4
Completeness [%]	96.7	90.2
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	310	MPD, KCl, MgCl2, Na cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K