2FCP
FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.550, 171.550, 87.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.28300 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1fcp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.400 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.078 * | 0.334 * |
| Number of reflections | 53749 | 2931 * |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 99.3 | 92.2 |
| Redundancy | 6.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 1% CIS-INOSITOL, 3% PEG200 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.4 |
| 3 | 1 | reservoir | PEG2000MME | 11 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | |
| 5 | 1 | reservoir | PEG200 | 3 (%) | |
| 6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
| 7 | 1 | reservoir | cis-inositol | 1 (%) |
