2FCO
Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | OXFORD |
| Wavelength(s) | 0.9787, 0.9784, 0.9521 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.886, 96.886, 114.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.990 - 1.400 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.089 | 0.411 |
| Number of reflections | 103958 | |
| <I/σ(I)> | 9.8 | 0.6 |
| Completeness [%] | 86.9 | 30.4 |
| Redundancy | 5.5 | 0.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15-175 PEG20000. 100mM Tris-HCl, , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15-175 PEG20000. 100mM Tris-HCl, , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
