2FCJ
Structure of small TOPRIM domain protein from Bacillus stearothermophilus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.617, 72.720, 116.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.300 |
R-factor | 0.19238 |
Rwork | 0.192 |
R-free | 0.21399 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.163 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 77810 | |
<I/σ(I)> | 76.8 | 2 |
Completeness [%] | 99.5 | 94.4 |
Redundancy | 15.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH6.0; 26% PEG3350; 0.2M Ammonium Sulfate; 18% glycerol; protein concentration 3.5mg/ml; micro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K |