2FCJ

Structure of small TOPRIM domain protein from Bacillus stearothermophilus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	36.617, 72.720, 116.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.300
R-factor	0.19238
Rwork	0.192
R-free	0.21399
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	77810
<I/σ(I)>	76.8	2
Completeness [%]	99.5	94.4
Redundancy	15.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES pH6.0; 26% PEG3350; 0.2M Ammonium Sulfate; 18% glycerol; protein concentration 3.5mg/ml; micro-seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K