2FCF
The crystal structure of the 7th PDZ domain of MPDZ (MUPP-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90010 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.122, 43.801, 50.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.990 - 1.760 |
| R-factor | 0.20937 |
| Rwork | 0.208 |
| R-free | 0.24396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n7f |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.990 | 1.850 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.073 | 0.096 |
| Number of reflections | 8968 | |
| <I/σ(I)> | 9.57 | |
| Completeness [%] | 95.0 | 96.3 |
| Redundancy | 6.84 | 6.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 30% mPEG 2K, 0.10M KSCN, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
