2FCB
HUMAN FC GAMMA RECEPTOR IIB ECTODOMAIN (CD32)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.830, 50.920, 80.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.740 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.27900 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.007 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.055 | |
| Number of reflections | 18009 | |
| Completeness [%] | 93.0 | 86.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.4 * | 20 * | 33% PEG 2000, 0.2M NAOAC, PH5.4, pH 5.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | MPS | 2 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | sodium azide | 0.02 (%) | |
| 5 | 1 | reservoir | PEG2000 | 33 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.2 (M) |
