2FCA

The structure of BsTrmB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-22
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	H 3
Unit cell lengths	178.830, 178.830, 41.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.233
Rwork	0.202
R-free	0.23300
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.044	0.028	0.318
Number of reflections	29105
Completeness [%]	99.7	99.8	97
Redundancy	5.3	5.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10 % glycerol, 10 % PEG4000, 200 mM KCl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	10 % glycerol, 10 % PEG4000, 200 mM KCl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	10 % glycerol, 10 % PEG4000, 200 mM KCl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K