2FC3
Crystal structure of the extremely thermostable Aeropyrum pernix L7Ae multifunctional protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-06 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.054, 49.188, 65.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.440 - 1.560 |
R-factor | 0.177 |
Rwork | 0.174 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xbi |
RMSD bond length | 0.014 |
RMSD bond angle | 1.419 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.050 | 1.600 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.025 | 0.289 |
Number of reflections | 13926 | |
<I/σ(I)> | 23.7 | 2.3 |
Completeness [%] | 81.5 | 15.8 |
Redundancy | 2.45 | 1.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 4000, 10% Isopropanol, 100 mM HEPES-KOH, pH 7.5, 1 mM ZnCl2 mixed 1:2 with 500 microMolar L7Ae protein, VAPOR DIFFUSION, HANGING DROP, temperature 298K |