2FBZ

Heme-No complex in a bacterial Nitric Oxide Synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-07-21
Detector	RIGAKU
Wavelength(s)	0.93
Spacegroup name	P 21 21 2
Unit cell lengths	79.995, 93.503, 62.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.23235
Rwork	0.231
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.136
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.900	1.900
Number of reflections	35595
Completeness [%]	99.0	93.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6.5	298	5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6.5	298	5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K