2FBZ
Heme-No complex in a bacterial Nitric Oxide Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-21 |
Detector | RIGAKU |
Wavelength(s) | 0.93 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.995, 93.503, 62.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.23235 |
Rwork | 0.231 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.136 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 35595 | |
Completeness [%] | 99.0 | 93.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 6.5 | 298 | 5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 6.5 | 298 | 5-10% PEG8K, NOC-12, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, temperature 298K |