2FBX
WRN exonuclease, Mg complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 80.806, 80.806, 93.174 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.700 - 2.200 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fbt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.700 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.365 |
Number of reflections | 17851 | |
<I/σ(I)> | 29.7 | 5.7 |
Completeness [%] | 87.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCL, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, pH 4.50, VAPOR DIFFUSION, SITTING DROP |