2FBX

WRN exonuclease, Mg complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 32 2 1
Unit cell lengths	80.806, 80.806, 93.174
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.700 - 2.200
R-factor	0.242
Rwork	0.242
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbt
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.056	0.365
Number of reflections	17851
<I/σ(I)>	29.7	5.7
Completeness [%]		87.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5		5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCL, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, pH 4.50, VAPOR DIFFUSION, SITTING DROP