2FBT
WRN exonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.269, 80.269, 93.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.870 - 2.050 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INITIAL MODEL BUILT FROM 2.5A SELENOMET MAD DATASET USED FOR MR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.870 | 2.180 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.331 | |
| Number of reflections | 21309 | |
| <I/σ(I)> | 20.8 | 3 |
| Completeness [%] | 99.7 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 277 | 5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCl, 100mM NaCl, pH7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservoir solution and 1ul EDTA additive. , VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 4.50 |
