2FBK

The Crystal Structure of HucR from Deinococcus radiodurans

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	98
Detector technology	CCD
Collection date	2004-08-08
Detector	MARRESEARCH
Wavelength(s)	1.380, 0.9795, 0.9798, 0.9500
Spacegroup name	P 61
Unit cell lengths	44.991, 44.991, 284.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.960 - 2.300
Rwork	0.239
R-free	0.28900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	14303
<I/σ(I)>	12.1
Completeness [%]	98.8	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	22% PEG 3350, 500 mM MgCl2, 100 mM Bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7	277	22% PEG 3350, 500 mM MgCl2, 100 mM Bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K