2FBH
The crystal structure of transcriptional regulator PA3341
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-12 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0093223, 1.007416, 0.97940 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.914, 82.914, 66.415 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.900 - 1.800 |
| R-factor | 0.19686 |
| Rwork | 0.195 |
| R-free | 0.23284 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 12313 | |
| <I/σ(I)> | 55 | 5 |
| Completeness [%] | 97.5 | 96.1 |
| Redundancy | 23 | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 0.1M (NH4)2SO4, PEG3350 20%, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
