2FBD
The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.4310 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.523, 79.435, 45.203 |
| Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.15565 |
| Rwork | 0.153 |
| R-free | 0.19802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hew |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.222 |
| Number of reflections | 19857 | |
| <I/σ(I)> | 23.8 | 5.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1% PEG 400, 0.1 M Na Hepes, 1.4 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
