2FBB
Crystal Structure Analysis of Hexagonal Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 10 |
| Detector technology | CCD |
| Collection date | 2004-06-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.637, 85.637, 67.932 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 1.460 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DPW.pdb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.470 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.038 | 0.884 |
| Number of reflections | 25570 | |
| <I/σ(I)> | 77.6 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 293 | 10% aqu. acetone solution saturated with sodium nitrate, 25mg/ml lysozyme, pH 8.4, batch, temperature 293.0K, pH 8.40 |
