2FBA

Glucoamylase from Saccharomycopsis fibuligera at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2002-07-09
Detector	MARRESEARCH
Wavelength(s)	0.834
Spacegroup name	P 21 21 21
Unit cell lengths	56.901, 85.696, 98.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.800 - 1.100
R-factor	0.14586
Rwork	0.145
R-free	0.16119
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ayx
RMSD bond length	0.007
RMSD bond angle	1.203
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.129
High resolution limit [Å]	1.100	1.100
Rmerge	0.059
Number of reflections	208793
<I/σ(I)>	16.9
Completeness [%]	93.5	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	295	50 mM acatate buffer, 15% PEG 8K, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K