2FBA
Glucoamylase from Saccharomycopsis fibuligera at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.834 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.901, 85.696, 98.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.800 - 1.100 |
| R-factor | 0.14586 |
| Rwork | 0.145 |
| R-free | 0.16119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.203 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.129 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.059 | |
| Number of reflections | 208793 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 93.5 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 50 mM acatate buffer, 15% PEG 8K, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
