2FB6
Structure of Conserved Protein of Unknown Function BT1422 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | H 3 |
| Unit cell lengths | 64.880, 64.880, 70.457 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.460 |
| R-factor | 0.1332 |
| Rwork | 0.132 |
| R-free | 0.16130 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.075 | 0.571 |
| Number of reflections | 19084 | |
| <I/σ(I)> | 30.8 | 1.99 |
| Completeness [%] | 99.2 | 90.1 |
| Redundancy | 7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 35% tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
