2FB6
Structure of Conserved Protein of Unknown Function BT1422 from Bacteroides thetaiotaomicron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97856 |
Spacegroup name | H 3 |
Unit cell lengths | 64.880, 64.880, 70.457 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.460 |
R-factor | 0.1332 |
Rwork | 0.132 |
R-free | 0.16130 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.233 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.490 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.075 | 0.571 |
Number of reflections | 19084 | |
<I/σ(I)> | 30.8 | 1.99 |
Completeness [%] | 99.2 | 90.1 |
Redundancy | 7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 35% tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |